Biography
Interests
Nemat Khansari
Department of Immunology, Medical University of Tehran, Tehran, Iran
*Correspondence to: Dr. Nemat Khansari, Department of Immunology, Medical University of Tehran, Tehran, Iran.
Copyright © 2021 Dr. Nemat Khansari. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Introduction
During December 2019, there were many reports of pneumonia type incidences caused by some unidentified
viral strain outbroke in Wuhan, China. The disease clinically resembled some new type of viral pneumonia and
Flu. After the isolation of the virus and several analyses of its genomic sequence, a novel strain of coronavirus
was identified which was designated as ‘Severe Acute Respiratory Syndrome-Related Coronavirus-2’ or
SARS-CoV-2. The respiratory diseases were caused by this newly discovered ‘SARS-CoV-2’ which was later
declared as ‘Coronavirus disease 2019’ (COVID-19) by World Health Organization.
COVID-19 virus is a member of a large group of viruses called corona viruses. These viruses are enveloped having single strand RNA. They called corona virus due to their crown-like morphology. They infect most of mammals and birds causing respiratory, enteric, hepatic, and neurologic diseases [1]. So far, seven species of corona viruses are known to cause human infection. Most of these human corona viruses have zoonotic origin. The four human corona viruses: HCoV-229E, HCoV-NL63, HCoV-OC43, and HCoV-HKU1 are endemic and prevalent [2]. Middle East respiratory syndrome corona virus (MERS-CoV) and newly identified sever acute respiratory syndrome corona virus (SARS-CoV2) are highly transmissible and pathogenic for human [3]. SARS-CoV2 has been identified as cause of corona virus disease 19 which is responsible for recent pandemic which occurred in 2019, hence the disease called COVID-19. Human corona disease (COVID-19) was first reported in Wuhan, China and rapidly spread all over the world infecting millions of people in every country of the world. SARS-CoV2 is member of the β-coronavirus family which has extensive genetic similarities with bat coronavirus suggesting that bats are the natural host of this virus [4].
When COVID-19 virus enters body via respiratory airway binds to the angiotensin converting enzyme 2(ACE2) through spike (S) of the virus that covers surface of virus [5]. Once virus bind to its receptor which is present on the cell, it penetrates into the cell and start replication process then newly developed virus exit the infect cells by budding outward then released for doing more invasion. ACE 2 is present on various organs cells like: heart, kidney, testis, and respiratory epithelial cells [6]. When a host cell that has ACE 2 on its surface meet the COVID-19 virus would get infected then following release of new virus from infected cell, the spike of COVID-19 virus appears on its surface (during the budding out of the new virus); thus, this cell also would be the target of immune response alike the free virus itself [7].
The pathogenesis of COVID-19 is as result of an abnormal host immune response due to local production of large number of inflammatory cytokines, chemokines and free radicals that can damage to lungs or other organs that have receptor for the virus [8]. COVID-19 infection induces pneumonia which is characterized by fever, cough, dyspnea, and bilateral infiltrates [9]. Edema and inflammatory clusters with fibrinoid material and multinucleated giant cells have also been reported in lungs of COVID-19 infected patients [10].
Immune response against COVID-19 start after virus invades host and bind to its receptor. Following viral penetrating cell and replication within cell, the virus is released where it is met by host’s innate immune system. T lymphocytes and dendritic cells are activated through pattern recognition receptors (PRRs) including C-type lectin-like receptors, Toll-like receptor (TLR), NOD-like receptor (NLR), and RIGl- like receptor (RLR). The virus induces the expression of numerous inflammatory factors, maturation of dendritic cells and synthesis of type 1 interferons (IFNs) which limits the viral spread and accelerates macrophage phagocytosis of viral antigens resulting in clinical recovery. However, the nucleus protein (N) od SARS-CoV2 can help the virus escape from the immune responses and overreaction of the immune response generates high levels of inflammatory mediators and free radicals. These processes induce sever local damage to lungs and other organs causing multi-organ failure and even death. The adaptive immune response also has major role in fighting against virus. T lymphocytes including CD4+ and CD8+ T cells play an important role in this defense. CD4+ T cells stimulate B cells to produce virus-specific antibodies IgM and IgG which can kill virus infected cells by antibody dependent cytotoxicity, but CD8+ T cells are able to directly kill virus infected cells [11]. CD4+ helper T cells also produce pro-inflammatory cytokines to help defending cells. In spite of all these host’s defense mechanisms, SARS-CoV2 can inhibit T cells functions by inducing programmed cell death (apoptosis) [12]. Cytotoxic lymphocytes (CTLs) and natural killer cells (NKs) are also important for control of viral infection; thus, decreased number of these cells may increase severity of diseases. In patients with COVID-19 the total number of NK and CTL are decreased in line with upregulation of NK inhibitory receptor CD94 [13]. Decreased number of CD4+ and CD8+ T cells in peripheral blood of COVID-19 infected patients increase proportion of HLA-DR (CD4+ 3.4%) and CD38 (CD8+ 39.4%) double-positive cells indicating highly activated cells [14]. In addition, it has been reported that activation of CD4+ and CD8+ are impaired which is evidenced by appearance of CD25, CD28, and CD69 expression on these T cell subsets [15]. These factors may account for the delay development of adaptive immune response and prolonged virus clearance in patients with sever cases of COVID-19 infection [16].
The cytokine storm syndrome is another manifestation id some of COVID-19 patients which is result of a hyperactivity of the immune system. In this condition the regulation of immune cells is often defective, resulting in the increased production and release of inflammatory mediators in blood stream by immune effector cells. These mediators are: INF-α, INF-δ, IL-1β, IL-6, IL-12, IL-18, IL-33, TNF-α, and TGF-β as well as certain chemokines such as CCL2, CCL3, CCL5, CXCL8, CXCL19 and CXCL10 [17]. Clinical symptom of cytokine storm is fever and confusion and laboratory tests show hyperferritinemia, lymphopenia, prolonged prothrombin time, elevated lactate dehydrogenase, elevated IL-6, elevated C-reactive protein, and elevated soluble CD25 [18].
Discussion
Since December 2019 that SARS-CoV2 was identified in several hospitalized patient in Wuhan, Hubei
Province in China, until now several thousand research articles have been published describing many aspects
of this highly transmissible and infectious virus that caused the viral pandemic of the current century. All
research aims were focused on mechanisms of viral spread, infectivity, diagnosis host defense, treatment,
and best strategy for vaccine development controlling the viral pandemic. In order to achieve most of these
goals, studying mechanism(s) of host defense has been very crucial. Today, scientist unveil a lot of important
information about immune response against COVID-19. Now we know that immune response to SARSCov2
begin with innate immune response get activated by free virus or infected cells and followed by
activation of humoral and cellular immune response in order to kill virus and/or infected cells of the various
organs that have ACE2 which act as COVID-19 virus receptor. The process of killing infected cell cause
damage of organ which is the cause of certain clinical symptoms like sever respiratory syndrome, heart
failure or kidney failure seen in many patients. Even though in patients with COVID-19 the white blood
cell count may vary between leukopenia, leukocytosis, and lymphopenia, it seems that white blood cell
counts in COVID-19 infected patients is very important indicator for prognosis of disease in patient [19].
It has been shown that lower lymphocyte counts indicated a poor prognosis. However, we do not know why
some people get infected but that do not show any clinical symptoms or many patients suffer from mild
infection the others very sever one. In addition, the reason for the resistance of children to COVID-19 is
also unknown. Even though many vaccines have been developed and we know both humoral and cellular
immune response are responsible for host defense but we do not know what titer of antibody provide
sufficient immunity against re-infection or even if cellular immunity provide long term immunity.
Bibliography
Hi!
We're here to answer your questions!
Send us a message via Whatsapp, and we'll reply the moment we're available!