Isolation and Molecular Characterization of Probiotic Bacteria and their Antagonistic Effect on Fish Pathogen Aeromonas Hydrophila

Samane Homaei, Farzane Farokhi^* & Alam Ara Gholamis

Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Sari Branch, Sari, Iran

Dr. Farzane Farokhi, Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Sari Branch, Sari, Iran.

Keywords: Probiotic; Bacteria; Aeromonas Hydrophila; PCR

Abstract

The term probiotic comes from Greek origin: pro and bios meaning “prolife”, which have used with different meanings over the years [1]. It was used since 1970s to introduce the supplements fed with useful microbes for human and animals [2]. The first study of probiotic bacteria from the aquaculture environment was reported in 1980s [3]. The use of probiotics for aquatic animals extremely demanded and must be harmless for aquaculture practices [4].
According to the reports of WHO, production of world aquaculture is noticeably growing and newly released FAO aquaculture statistics has recorded of 106 million tons in live production in the year 2015. Within 15 recent years, the aquaculture portion to the world production of aquatic animals (both captured and farmed) has increased from 25.7% in 2000 to 45.3% in 2015. Among all farmed aquatic animals for human feeding, aquaculture provided average 10.42kg of human eating food fish in 2015, which has been raising up to 0.28kg, from 10.14kg in 2014 and mainly by farming the finfish (trout) all around the world [5]. Fish diseases are major problem to be concerned and need urgent microbial control strategies in aquaculture because they have a great effect on aquaculture production, trading and occurring the antibiotic resistance [2].

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