Quantification of Viable Bacteria in Bacterial Cultures

Sabiha Habib & Ambreen Ahmed^*

Department of Botany, University of the Punjab, Quaid-e-Azam Campus, Lahore 54590, Pakistan

Dr. Ambreen Ahmed, Department of Botany, University of the Punjab, Quaide-Azam Campus, Lahore 54590, Pakistan.

Keywords: Bacterial Quantification; CFU; Bacillus Pumulis; Bacillus Cereus

Abstract

Bacteria are ubiquitous and can be located everywhere on the planet. The quantification of bacteria and their growth pattern studies in different biological samples is an essential part of microbiology and being used in various researches. Quantifying bacterial evaluation has particular importance in food, water or clinical samples. A variety of techniques have been introduced to determine the viable bacterial counts in sample cultures however CFU count by serial dilution method is the most applied and error-free methodology. Altogether, there is a correlation between bacterial quantification and optical density of bacterial cultures. The aim of the present work is to provide a very simple and cost-effective scientific approach to count bacterial colonies of selected bacterial isolates [Bacillus pumulis (ALa), Bacillus atrophaeus (BL2), Staphyllococcus lentus (E3), Bacillus cereus (AR), T2aii and W6ii] and to correlate with OD600 measurements of those bacterial cultures indicating the efficient and competitive bacterial strains in respect of their growth pattern and rapid multiplication.

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