Nematicidal Activity of Camellia Sinensis (Green Tea) Ethyl Alcohol Crude Extract

Nighat Sultana^1*, Muhammad Saleem Qazi¹ & Alay Khan²

¹Pharmaceutical Research Center, Pakistan Council of Scientific and Industrial Research (PCSIR) Laboratories Complex, Karachi-75280, Pakistan
²Crop Diseases Research Institute, PARC, University of Karachi, Karachi-75270 - Pakistan

Nighat Sultana, Pharmaceutical Research Center, Pakistan Council of Scientific and Industrial Research (PCSIR) Laboratories Complex, Karachi-75280, Pakistan.

Keywords: Camellia Sinensis; Carbofuran Standard; Helicotylenchus Indicus; Xiphinema Americanum; Xiphinema Index; Nematicidal Activity

Abstract

The nematicidal effect of ethyl alcohol extract of Camellia sinensis (Green tea) leaves were tested against Helicotylenchus indicus, Xiphinema americanum and Xiphinema index at the concentrations of 1.0 and 0.1%, extract was used in distill water and observation taken at different time periods and compared with chemical neamticide carbofuran. There were four replicates of each test conducted by taking conc. percentage 1.0 and 0.1 and mortality was recorded. Nematicidal mortality was 100% with carbofuran chemical nematicide at different time periods at same time ethanol extract also shown nematicidal property at different time periods on above nematodes and with the use of 1% concentration of Camellia sinensis leaves ethyl alcohol extract applied on Helicotylenchus indicus, after 1 hour and 24hours and observed 50 mortality in 1hour and 22.5 mortalities during 24hours and 0.1% concentration of extract application 2.5 for 1hour and 6.25 for 24hours. Same nematicidal activity test conducted on Xiphinema americanum and Xiphinema index and recorded mortality level at 1% extract concentration 50 and 46.25, where 0.1 extract concentration shown 0.0 and 6.25 mortalites in 1hour, 24hour extract application of 1% and 0.1% are as 63.25, 0.0 and 20.0, 0.0. The plant is economic importance having nematicidal value. These results showed that the extract obtained from Camellia sinensis have potent nematicidal activity and may be responsible for the antinematicidal activity of whole plant.

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