Evaluation of Prothrombin Time and Activated Partial Thrombin Time in Albino Rats Fed with Different Concentration of Used Engine Oil

Chukwurah Ejike Felix¹, Obeagu Emmanuel Ifeanyi^2* & Chukwu Ngozi Bethel³

¹Department of Haematology and Immunology, Faculty of Clinical Medicine, Ebonyi State University, Abakaliki, Nigeria
²Department of Medical Laboratory Science, Faculty of Health Sciences, Imo State University, Owerri, Nigeria
³Department of Medical Laboratory Science, Faculty of Health Sciences, Ebonyi State University, Abakaliki, Nigeria

Obeagu Emmanuel Ifeanyi, Department of Medical Laboratory Science, Faculty of Health Sciences, Imo State University, Owerri, Nigeria.

Keywords: Activated Partial Thrombin Time; Engine Oil; Prothrombin Time

Abstract

This study aimed to evaluate the prothrombin Time and Activated partial thrombin Time (APTT) of Albino rats, fed with varied concentrations of used engine oil. A total of twenty Wister Albino rats aged 10 (ten) weeks and weighing 97-99kg were used. The rats were divided into five groups containing 4 rats each. Orally administered doses were 0.2ml, 0.4ml, 0.6ml. 1ml and in feed are 1ml. 2ml, 3ml and 4ml of used engine oil corresponding to groups B, C, D and E, respectively for 28 days while group A (control) received uncontaminated food and water. The sample collection was through retro-orbital plexus, the sample were collected into a plain plastic tube containing trisodim citrate citrate anticoagulant in the ratio 9:1 (2ml of blood in 200ul trisodium citrate) and calcium chloride. The Prothrombin time samples were analysed by platelet poor plasma concentrate method and APTT with Kaolin reagent. The Analysis of variance (Anova) expressed in mean+ standard deviation showed dose dependent significant increase in the prothrombin Time 0.2ml dose (15.00±11.63), 0.4ml (33.00±3.06). 0.6ml (34.00±3.50) and APTT 0.2ml (39.00±2.63). 0.4ml (105.00±5.03), 0.6ml (108.00±2.38) compare to the control group.

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