Association of Glucosyltransferase with Streptococcus Mutans and DMFT in Caries Active Children

Anoop Kumar¹, Nithya Jagannathan^2* & Yekkoluku Nagaranjitha²

¹Department of Oral and Maxillofacial Pathology, PSM College of Dental sciences and Research, Trichur, Kerela, India
²Department of Oral Pathology, Saveetha University, India

Dr. Nithya Jagannathan, Department of Oral Pathology, Saveetha Dental College, Chennai, India.

Keywords: Dental Caries; Glucosyltransferase; DMFT; Oral Microbiota; Streptococcus Mutans

Abstract

Objective
The aim of the study was to establish a relationship of dental caries with the GTF and streptococcus mutans count to determine the caries active individuals.
Material and Methods
The study comprised of 40 children, of which 20 were clinically diagnosed as having early carious lesions and the rest were clinically free of caries. About 2ml of unstimulated saliva was collected from the study group and the control group. The salivary sample was uniformly mixed and about 1.5ml of saliva was streaked on the Blood agar and chocolate agar supplemented with bacitracin, using a 4mm inner diameter inoculation loop. The MSR agar plates were incubated anerobically for 48 hours at 37 degree celcius. Following the incubation, counts were made from the colonies which had morphologic characteristic of Streptococcus mutans on the MSB agar. Glucosyltransferase activity was measured using DNA assay.

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