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In Vitro Activity of Bacteriophage and Chitosan Against Sea Water Isolated Bacteria

Asdren Zajmi1*, NurAfiqah Azmi1 , Yasohdha Anne Sundraraj1 & Safaa Saud, N.2

1Department of Diagnostic and Allied Health Science, Faculty of Health and Life Science, Management & Science University, 40100 Shah Alam, Selangor, Malaysia
2Faculty of Information Sciences and Engineering, Management & Science University, 40100 Shah Alam, Selangor, Malaysia

Dr. Asdren Zajmi, Department of Diagnostic and Allied Health Science, Faculty of Health and Life Science, Management & Science University, 40100 Shah Alam, Selangor, Malaysia.

Keywords: Sea Water Isolated Bacteria; Phage Therapy; Chitosan; Antibacterial Activity

Abstract

The primary cause of morbidity and mortality is the soft tissue infections after bacterial infection with saltwater exposure. Bacteriophages were being considered to be bacteria that absorb viruses, so active bacteriophages can be applied theoretically to prevent bacterial infections. Chitosan, a partly deacidified natural polymer derived from a crustacean shell or fungus, which has antimicrobial potential. The present study aims to investigate bacterial and chitosan antibacterial activity against isolates of marine water. Five lytic phages in the allocated area of Shah Alam, Malaysia were isolated from marsh water and tested against five different seawater-isolated bacterial strains. Phage and disk diffusion experiments are used to assess the sensitivity profiles of the five isolated strains of marine water. The five isolates of bacteria, including small plaques and chitosan, were found to be susceptible to isolated phage.

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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