In Vitro Investigation of Antibacterial Activity of Combined Leaves Extracts of Alstonia scholaris and Fernendoa adenophylla against Urinary Tract Infection Pathogens

Muhammad Saqib Ishaq^*, Aqdas Zoreen, Abdul Rehman, Sidra Farooq & Ghadir Ali

Department of Microbiology & Biotechnology, Abasyn University, Peshawar, KP, Pakistan

Dr. Muhammad Saqib Ishaq, Department of Microbiology & Biotechnology, Abasyn University, Peshawar, KP, Pakistan.

Keywords: Urinary Tract Infection; Pathogens; Bacterial Strains

Abstract

In the present research study, the antibacterial activity of combined leaves extracts of Alstonia scholaris and Fernandoa adenophylla against urinary tract pathogens was investigated. A total of ten different bacterial strains (E. coli, Klebsiella spp, Proteus spp, Salmonella spp, Morganella morginii, Pseudomonas aeroginosa, Providencia spp, Staphylococcus aureus and Streptococcus pyogene ) were isolated from urine samples. Through disc diffusion method, seven different antibiotics were used against the isolated pathogens to determine their culture sensitivity patterns. Among the seven used antibiotics, Meropenem was most effective and produced the largest ZI 44mm, and was 100?tive followed by Azithromycin (90%), Pipracillin (90%), Ciprofloxacin (90%), Amikacin (90%) and Nalidixic acid (80%), while Doxycycline showed average activity and was resistant to five tested bacterial strains. Well diffusion method was used to evaluate the antibacterial activity of leaves extracts of both plants. For this purpose, extracts of both plants were mixed together and five combinations were obtained and were tested against the isolated bacterial strains. Among the five combinations, the combination of (n-hexane extract of F. adenophylla + Water extract of A. scholaris) showed broad-spectrum antibacterial activity against all the tested bacterial strains and produced the highest zone of inhibition 18.5 mm against Salmonella spp, whereas the combination of Methyl alcohol extract of A. scholars mixed with n-hexane extract of F. adenophylla, showed no activity. The other three combinations showed average activity against the tested bacterial strains. From the present study it can be concluded that the n-hexane extract obtained from the leaf of F. adenophylla mixed with the water extract obtained from the leaf of A. scholaris plant, was found to have antibacterial activity against UTI causing bacterial pathogens. So further study in this regard will be definitely helpful in producing plant-based antimicrobial compounds to replace synthetic antibiotics and suppress the emergence of antibiotic resistance strains.

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