Isolation and Identification of Bacteria from Wound Infection from Patients Attending Orile-Agege and Ifako General Hospitals, Lagos State, Nigeria

Ajayi, J. B.^1*, Taiwo, S. S.², & Ajayi, O. A.³

¹Ogun State Institute of Technology, Igbesa, Nigeria
²Orile-Agege and Ifako General Hospitals, Lagos State, Nigeria
³Lagos State Unversity, Lagos, Nigeria

Dr. Ajayi, J. B., Ogun State Institute of Technology, Igbesa, Nigeria.

Keywords: Wound; Exudates; Diabetic Ulcer

Abstract

Background
Infection caused by wounds has continued to be a problem in most hospitals where treatment of infection has become generally by observation.
Methods
The isolation and identification of bacteria associated with sepsis was carried out on 150 patients attending Orile-Agege and Ifako- Ijaiye, Lagos, Nigeria. Samples such as wound pus were collected with striates that has not been contaminated with bacteria. Patients with diabetic ulcer, surgery wounds, sickle cell ulcers and accidental wounds were used. Sterile swab sticks were used to aspirate the wound exudates and to swab the wound. Different wound infections were also noted. Samples collected were streaked on differential media such as MacConkey agar, blood agar, CLED agar and chocolate agar. Further examination was done such as wet preparation, gram staining, coagulase test and catalase test for foams confirmation.

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