CPQ Medicine (2021) 11:5
Research Article

Antibacterial Activities of Widely Spread Taraxacum Officinale Dandelion in Al-Qadmous, Syria as Potential Therapeutic Strategy for Antibiotic Resistant Bacteria

Rim Harfouch, M.1*, Manal Darwish2, Soumya Ghosh3, Ranim Ahmad2, Rasha Kherbeik2, Nermin Khateb2 & Conrad Chibunna Achilonu3

1Department of Microbiology and Biochemistry, Faculty of Pharmacy, Al Andalus University, Tartous, Syria
2Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Al Andalus University, Tartous, Syria
3Department of Genetics, Faculty of Natural & Agricultural Sciences, University of the Free State, P.O. BOX 339, Bloemfontein 9300, Free State, Republic of South Africa

*Correspondence to: Dr. Rim Harfouch, M., Department of Microbiology and Biochemistry, Faculty of Pharmacy, Al Andalus University, Tartous, Syria.

Copyright © 2021 Dr. Rim Harfouch, M., et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: 20 February 2021
Published: 11 March 2021

Keywords: Antibacterial Activity; Taraxacum officinale; Ethanolic Extract; Bacterial Stains; Antibiotics; Antibiotic Resistant


Medicinal plants perform an important role within the treatment of upper respiratory illness such as sore throat, cold and flu. The study aimed to highlight the biological significance of antimicrobial activities exhibited by the ethanolic extract of Taraxacum officinale (dandelion) leaves and roots against the bacterial strains. The leaves and roots of T. officinale where collected from Qadmous area, Tartous, Syria, and were extracted with ethanol and tested for their antimicrobial activities against the bacterial strains of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and the pathogenic strain of Streptococcus pneumoniae along with trimethoprim/sulfamethoxazole (TMP/SMX) antibiotics (positive control). The root extracts exhibited the maximum inhibition against S. aureus, P. aeruginosa and E. coli with zone of inhibitions measuring 24mm, 17mm and 12mm respectively, in comparison to the leaves extracts that exhibited antibacterial activities against S. pneumoniae (11mm) and P. aeruginosa (14mm). Notably, TMP/SMX antibiotics were observed to have larger antibacterial activities against E. coli (31mm) and S. aureus (30mm). These findings stem the possibility of using the extracts of T. officinale, especially the root extract as a co-factor to antibiotics in order to eliminate multiple antibiotics resistant bacteria, especially P. aeruginosa and S. aureus.

List of Abbreviations

MRSA : Methicillin-resistant S. aureus
TMP/SMX : Sulfamethoxazole and Trimethoprim
TPC : Total phenolic components

The World Health Organisation evaluated that up to 80% of the world population often uses conventional remedies [1], with more than 35,000 plant species are utilised as medicine to alleviate illness in several human customary communities [2]. Taraxacum officinale. L. (dandelion) is a perennial weed plant from the Asteraceae (Compositae) family mostly found in the temperate area of the Northern hemisphere and several parts of the world [3]. However, the plant has medicinal significance [4], and is widely used across Syria [5]. Noticeably, the roots of Taraxacum officinale are maximally used for medicinal studies, however in many cases the leaves and whole plant are also used [6]. Taraxacum officinale contains therapeutic properties because of the presence of phytochemicals such as tannins, flavonoids that exhibits healing activities and are widely studied in various areas of human health treatment [7-10]. Compounds such as tannins [11] and flavonoids are known as anticancer agents [12], and joint pain inhibitor [13,14] respectively. This plant contains high concentrations of potassium, which is crucial to the process of diuresis and calcium, and reduces the feeling of numbness in the limbs [15]. Taraxacum also has anti-inflammatory properties related to the compound taraxasterol and the prevention of heart disease by reducing cholesterol levels [16]. Various parts of T. officinale have been investigated for their antioxidant properties [17, 18], anti-quorum sensing activities [19], anti-inflammatory properties related to compound taraxasterol and the prevention of heart disease by reducing cholesterol levels [20,21].

Figure 1: Syrian Taraxacum officinale plant depicting its different organs. Leaves have been used in our study as exhibited by a rectangle box

Additionally, T. officinale plant has the ability to get rid of heat and toxins in humans, and thus, reduce swelling, inflammation, excessive production of urine and the flow of bile from the liver [22]. A previous study [23] has reported the effects of T. officinale extracts on oxidative stress, inflammation, and lipid profile in C57BL/6 mice fed atherogenic diet and therefore there could be a possibility that plant could reduce the risk of atherosclerosis in humans. Therapeutically, T. officinale relieves cold symptoms and sore throats during flu season. For example, a study [24] demonstrated how T. officinale extracts can inhibit both A/ PR/8/34 and WSN (H1N1) influenza viruses by inhibiting viral nucleoprotein synthesis and polymerase activity. The authors further investigated the characterisation of the active compounds of the extracts and their specific mechanism against influenza virus. Apart from their medicinal importance, T. officinale is often used as nutritious food and beverage [6]. The leaves are used in the salads, sandwiches, tea and often cooked as vegetables. The roots are often substituted for coffee and flowers for wine and schnapps [6]. Moreover, a study [25] has shown that 100g of dry matter of T. officinale Weber (dandelion) contains a total dietary fiber 47.80g, ash 14.55g and proteins 15.48g.

The antimicrobial properties of T. officinale plant extracts have been reported earlier [26,27] and in particular the root’s ethanolic and methanolic extracts was found to be more effective in suppressing the growth of Staphylococcus aureus, methicillin-resistant S. aureus (MRSA) and Bacillus cereus strains [28,29].

At the other end, over dosage of antibiotics are often linked to side effects on hosts which involve hypersensitivity, immunosuppression and allergic reactions [30]. These rapid uses of antibiotics lead to the development of antibiotic resistant microbial strains, causing rapid failure of chemotherapeutics, significantly influencing the use of biological bioactive molecules to alleviate the human infections [31]. Therefore, information about antimicrobial potentials of plant extracts on different microbial strains are important in order to evaluate their potentials as antimicrobial agents especially against multidrug resistant and pathogenic microbial strains [32-37].

Therefore, the present study investigated the in vitro assay of the antibacterial activity of ethanol extracts of leaves and roots of Syrian T. officinale plant against Gram-positive (Staphylococcus aureus, Streptococcus pneumoniae) and Gram-negative (Escherichia coli, Pseudomonas aeruginosa) bacteria. To the best of our knowledge, this is the first study conducted on the antimicrobial activities of T. officinale plant extracts in Syria.

Materials and Methods

Sample Collection
Fresh leaves and roots of T. officinale were collected from across Al-Qadmous countryside in February, 2019 and was transported to the Department of Microbiology Al Andalus University, Tartous, Syria. The plant materials were surface-sterilised by washing with tap water, disinfected in 1% bleach [sodium hypochlorite (NaOCl)] for 3 - 5 min, rinsed with sterilised distilled water (dH2O) for 2 min and then dried using laboratory tissue paper.

Plant Extract Preparation
All plant parts were air dried in shade for two weeks at room temperature 20-25°C followed by in an oven at 40°C for 15 minutes every day for a week until the stability of weight was attained. The dried leaves and roots were pulverized using a mechanical grinder. The plant extracts were prepared by cold extraction method using ethanol solvent [38]. Approximately 10g of each the powdered plant materials were soaked in 70mL of ethanol and left for 2 days at room temperature in order to diffuse out the secondary metabolites into the solvent. Further the solution was filtered with Whatman filter paper No. 2 (Munktell & Filtrak GmbH, Barenstein, Germany), and the filtrates were concentrated at 40°C for 3 days at 25 rpm on a rotary evaporator (Laborota 4000-efficient, Heldolph, Germany). The residue was collected and stored at 4°C until further used.

Figure 2: Schematic representations of the experimental methodologies followed

Antibacterial Activity Assay
The bacterial strains (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa) and the pathogenic strain (Streptococcus pneumonia) were procured from the laboratory section of Tishreen University Hospital, Latakia, Syria and maintained on nutrient agar slants at 4°C. Each of the strains was streaked to a nutrient agar plate and incubated for 5 days at 37°C. A single pure colony of the respective bacterial strains was further inoculated into 5mL of Nutrient Broth (NB) and incubated at 37°C for 5 days. Following the incubation, the bacterial cultures were adjusted to a concentration of 106cells/mL for the antimicrobial assay.

The ager disc diffusion method [39] was implemented to determine the antibacterial activities of the ethanolic T. officinale extracts. The grown bacterial cultures were inoculated at a concentration of 106cfu/ mL in 20mL of molten agar media with a gentle shaking and poured in a Petri dishes (100mm x 15mm) and air-dried under laminar air flow (Esco Technologies, Pennsylvania, USA). The filter paper discs (6mm in diameter) were infused with 10μL ethanolic plant extracts, air dried and laid down on the agar media plate inoculated with the bacterial culture. The plates were incubated under aerobic conditions at 37°C for 48h. The antimicrobial activities were measured as the zone of clearance around each bacterial colony by subtracting the size of the infused disk from the zone of clearance observed.

However, the active components isolation and characterisation of the plant ethanolic extracts have not been performed in this study.


Antibacterial Activity
The antibacterial activity of ethanolic extracts of leaves and root of T. officinale with the standard drugs (TMP/SMX) showed from low to no inhibitory activities against all the tested bacterial strains (Table 1, Figure 3). The root extracts exhibited maximum inhibitory efficacy with zone of clearance measuring 24mm, 17mm and 12mm against S. aureus, P. aeruginosa and E. coli respectively while the leaves extract showed 14mm and 11mm against P. aeruginosa and S. pneumonia respectively. TMP/SMX (0.01mg/μL; positive control) showed strong antimicrobial effects against E. coli and S. aureus with zone of inhibition measuring 31mm and 30mm respectively while S. pneumoniae and P. aeruginosa showed no susceptibility towards the positive control, indicating that these microbes were found resistant to the drug. The negative control (water) does not exhibit any antimicrobial activities.

Table 1: Antibacterial activity of ethanolic extract of T. officinale leavess and roots against bacterial strains. +/- : denotes Gram positive / negative, NZI: No zone of inhibition

Figure 3: Antimicrobial activities (zone of inhibition values) of ethanolic extract of T. officinale leaves and root, Trimethoprim/ sulfamethoxazole (0.01 mg/⎛L; positive control) tested against different bacteria strains.

In recent years, there has been a decline in microbial susceptibility to the existing antimicrobial agents liable for drug resistance in hospitals and in communities causing a global epidemic of antibiotic resistance, leading to an ecological disaster of unknown consequence [40,41]. Numerous studies [42-45] have divulged the importance for new antimicrobial agents to replace the arsenal of anti-infective agents. However, owing mostly to the financial glitches, a failure of antibiotic discovery remained unnoticed, and thus, no new classes of antimicrobial agent were expected to be in use in 20 years [40,42]. For this reason, the development and research into naturally derived compounds (polyphenolics and brassinosteroids) from plants and thus, their wide-range of biological properties and bioactive constituents were proven to be useful against many disease causative agents [46].

The current study explored the antibacterial activities of ethanol extracts of roots and leaves of T. officinale against subcultures strains of S. aureus, S. pneumoniae, E. coli and P. aeruginosa. T. officinale exhibited to have antibacterial activity for both the root and leaves extracts. In consistent to the present study, a previous study has also shown the antimicrobial activities of seven herbal extracts of T. officinale against the Candida strains [47]. Furthermore, another study has reported hydroethanol extracts of T. officinale showed about 26% inhibition against H. pylori [48]. The present study revealed that the root extracts showed stronger antagonistic activities in comparison to leaves extracts. However, the zones of inhibition exhibited by TMP/ SMX against S. aureus and E. coli were more in comparison to the root extracts against the microbes. These findings were in accordance with earlier studies where the antimicrobial activities of Taraxacum extracts at 0.1mg/disc against E. coli, P. Vulgaris, S. aureus, and B. subtilis were relatively lower (zone of inhibition diameter =7.12 and 19.4mm) in comparison to gentamicin and tetracycline (18.9-38.8mm) [49,50].

Nonetheless, the root extracts gave a specific efficacy of 17mm inhibition diameter against Pseudomonas aeruginosa which is known for its antibiotic resistance and was not sensitive to the reference antibiotic (TMP/SMX). Also, root extract has an acceptable efficacy towards E. coli. Conversely, the root extract did not show any efficacy towards pulmonary bacteria Streptococcus pneumoniae in comparison to the leaves extract. The antibacterial activities of the root extracts against the antibiotic resistant strain could be attributed to the high content of phenolic compounds (secondary metabolites) in the roots in comparison to the leaves or other plant organs [17,28,51,52]. For example, a previous study tested the antimicrobial properties of crude and dialysed extracts from T. officinale root, where it has been further characterised by the presence of two hydroxyl fatty acids (NPF406) and three phenolic based compounds (NPF5) [28]. These compounds are likely to be responsible for the antimicrobial activity against S. aureus and B. cereus, thus, suggesting the use of dandelion root as a source of natural antimicrobial compounds. Furthermore, another study evaluated the total phenolic components (TPC) in T. officinale, where the highest phenolic contents obtained was in hydro-alcoholic extract (691.6mg/g GAE), thus, indicating significant role as antimicrobial agents [53].

Secondary metabolites from natural plant products are now the source for drug development. As they are produced in the living systems, it has shown more similarities to drugs and biologically compactible than synthetic drugs [54]. For instance, plant-derived drugs for anti-cancer are naturally derived compounds from plants, which are non-toxic to normal human cells, and thus, can be lead into clinical trials for further therapeutic development [55]. However, plant medicines can also function as models for pharmacologically active compounds that may possess higher activity and less toxicity than their synthetic counterparts [56,57]. According to a previous study [58], the globally control of helminthic disease in humans using synthetic drugs are less effective, and also causing numerous side effects. Additionally, the proceeded employment of synthetic anthelmintic/larvicidal drugs show major resistant drug problem in diseases caused by parasites. Olive leaves extract is proven to cure upper respiratory illness such as nasal obstruction, sore throat, tonsillitis, and common cold [59]. The study reported that olive extract comprises of polyphenols, significantly oleuropein and hydroxytyrosol; having antiviral, antibacterial, anti-inflammatory and antioxidant properties which reduces the rate of these upper respiratory illness. Nonetheless, our study was the first where ethanolic T. officinale extracts were used to determine the antibacterial activity against bacterial strains in Syria. The importance of these results stem from the possibility of using the extracts of T. officinale, especially the root extract as a co-factor to antibiotics to eliminate multiple antibiotics resistant bacteria, especially P. aeruginosa and S. aureus.

Using diverse natural plant compounds as antimicrobial agents is an intriguing approach for discovering bioactive products used in alleviating sicknesses, in particular, cold, sore throat and flu. However, owing to the fact that plants are widely rich in variety of secondary metabolites, such as flavonoids, alkaloids, tannins and terpenoids, which have been found in vitro to have antimicrobial properties is important to plant medicinal research. Interestingly, our results for this study is the first documented report in Syria to the best of our knowledge. However, the results demonstrate that the ethanol root extract of T. officinale and TMP/ SMX antibiotics is one of the drug that could be invested to take advantage of its antimicrobial compounds, especially as a co-factor for the treatment of bacterial infections resistant to antibiotics. This would assist to replace drugs to which bacteria have evolved resistance by encouraging traditional medicines. Moreover, further research on T. officinale by using different solvents may increase the efficacy rate of the plant product. Also, these findings can be helpful for the development of natural phytotherapeutic agents against diseases of humans and animals. The future prospects of this study should endorse to test other multidrug resistant bacterial strains such as Vancomycin-Resistant Enterococci and Extended-spectrum β-lactamase (ESBLs) producing Gram-negative bacteria. Furthermore, possibly it can also be extended to antiviral tests especially against virus such as sars2-cov-2 virus causing COVID-19. Additionally, the study can also be performed for cytotoxicity test against the animal cell lines (in vitro) and in vivo on model animals. Finally, this study will shed light on an important Mediterranean plant spread over in Syria and other parts of the Northern hemisphere in the hope that the research and studies will be completed in forthcoming projects.

Author Contributions
SG and RMH conceived the study and designed the experiment. RMH, MD, RA, RK and NK conducted all the experiments. SG and CCA drafted the manuscript. SG and RMH read and edited the manuscript.

We are thankful to Dr. Swagata Ghosh, Assistant Professor of English, Kumaraguru College of Arts and Science, Coimbatore, India for editing this manuscript.

Grant Support
The authors declare that there was no grant support provided for this study.

Conflict of Interests
The authors declare no financial interest or conflict of interest.


  1. Arunkumar, S. & Muthuselvam, M. (2009). Analysis of phytochemical constituents and antimicrobial activities of Aloe vera L. against clinical pathogens. World Journal of Agricultural Sciences, 5(5), 572-576.
  2. Philip, K., Malek, S. N. A., Sani, W., Shin, S. K., Kumar, S., Lai, H. S., et al. (2009). Antimicrobial activity of some medicinal plants from Malaysia. American Journal of Applied Sciences, 6(8), 1613-1617.
  3. Wigg, F. H. (2020). Plants Profile for Taraxacum officinale (common dandelion). Natural Resources Conservation Service PLANTS Database. 2020.
  4. Maggi Dandelion, F. (2018). In: Nabavi SM, Silva AS, editors. Nonvitamin and Nonmineral Nutritional Supplements. Cambridge, UK: Elsevier, (Pp. 203-204).
  5. Khouli, L. (2020). Syriatimes.sy - Hindbeh (sautéed dandelion greens). Syria, 2020.
  6. Murray, M. T. (2020). 117 - Taraxacum officinale (Dandelion). In: Pizzorno JE, Murray MT, editors. Textbook of Natural Medicine (Fifth Edition). St. Louis (MO): Churchill Livingstone. (Pp. 876-8.e1).
  7. Valenzuela, M. E. M., Peralta, K. D., Martinez, L. J. & Maggi, R. C. (2017). Taraxacum Genus Extract Experimental Approaches. Herbal Medicine: Intech open., (Pp. 274-301).
  8. Clare, B. A., Conroy, R. S. & Spelman, K. (2009). The Diuretic effect in human subjects of an extract of Taraxacum officinale Folium over a single day. J Altern Complem Med., 15(8), 929-934.
  9. Martinez, M., Poirrier, P., Chamy, R., Prufer, D., Schulze-Gronover, C., Jorquera, L., et al. (2015). Taraxacum officinale and related species-An ethnopharmacological review and its potential as a commercial medicinal plant. Journal of Ethnopharmacology, 169, 244-262.
  10. Al-Khuzaay, H. M. & Aljuraisy, Y. (2020). The activity of aqueous extracts of leaves and roots of Dandelion on cancer cell lines. Plant Archives, 19(2), 3933-3941.
  11. Okuda, T. & Ito, H. (2011). Tannins of constant structure in medicinal and food plants-hydrolyzable tannins and polyphenols related to tannins. Molecules, 16(3), 2191-2217.
  12. Yildirim, I. & Kutlu, T. (2015). Anticancer agents: Saponin and tannin’. International Journal of Biological Chemistry, 9(6), 332-340.
  13. Williams, C. A., Goldstone, F., Greenham, J. (1996). Flavonoids, cinnamic acids and coumarins from the different tissues and medicinal preparations of Taraxacum officinale. Phytochemistry, 42(1), 121-127.
  14. Tita, B., Bello, U., Faccendini, P., Bartolini, R. & Taraxacum officinale, W. (1993). Pharmacological effect of ethanol extract. VI Congress of the Italian Society of Pharmacognosy Rome, Italy: Academic Press; 1993.
  15. Yang, R., Ling, J., Li, X., Zeng, F., Li, S., Zhang, X., et al. (2013). Effects of Taraxacum Polysaccharides on growth performance and immune function of growing meat rabbits from weaner to 3 months of age. Chinese Journal of Animal Nutrition, 25, 11-14.
  16. Kim, Y. C., Rho, J., Kim, K. T., Cho, C. W., Rhee, Y. K. & Choi, Y. K. (2008). Phenolicacid contents and ROS scavenging activity of Dandelion (Taraxacum officinale). Korean Journal of Food Preservation, 15(3), 325-331.
  17. Jedrejek, D., Lis, B., Rolnik, A., Stochmal, A. & Olas, B. (2019). Comparative phytochemical, cytotoxicity, antioxidant and haemostatic studies of Taraxacum officinale root preparations. Food Chem Toxicol., 126, 233-247.
  18. Huber, M., Triebwasser-Freese, D., Reichelt, M., Heiling, S., Paetz, C., Chandran, J. N., et al. (2015). Identification, quantification, spatiotemporal distribution and genetic variation of major latex secondary metabolites in the common dandelion (Taraxacum officinale agg.). Phytochemistry, 115, 89-98.
  19. Bacha, K., Tariku, Y., Gebreyesus, F., Zerihun, S., Mohammed, A., Weiland-Brauer, N., et al. (2016). Antimicrobial and anti-Quorum Sensing activities of selected medicinal plants of Ethiopia: Implication for development of potent antimicrobial agents. BMC Microbiology, 16(139).
  20. Esatbeyoglu, T., Obermair, B., Dorn, T., Siems, K., Rimbach, G. & Birringer, M. (2017). Sesquiterpene lactone composition and cellular Nrf2 induction of Taraxacum officinale leaves and roots and Taraxinic Acid beta-d-Glucopyranosyl ester. J Med Food., 20(1), 71-78.
  21. Wirngo, F. E., Lambert, M. N. & Jeppesen, P. B. (2016). The physiological effects of Dandelion (Taraxacum Officinale) in Type 2 Diabetes. Rev Diabet Stud., 13(2-3), 113-131.
  22. Sweeney, B., Vora, M., Ulbricht, C. & Basch, E. (2005). Evidence-based systematic review of dandelion (Taraxacum officinale) by natural standard research collaboration. J Herb Pharmacother., 5(1), 79-93.
  23. Kim, J. J., Noh, K. H., Cho, M. Y., Jang, J. Y. & Song, Y. S. (2007). Anti-oxidative, anti-inflammatory and anti-atherogenic effects of dandelion (Taraxacum officinale) extracts in C57BL/6 mice fed atherogenic diet. Faseb J., 21(6), A1122-A.
  24. He, W., Han, H. M., Wang, W. & Gao, B. (2011). Anti-influenza virus effect of aqueous extracts from dandelion. Virol J., 8(538).
  25. Escudero, N. L., De Arellano, M. L., Fernández, S., Albarracín, G. & Mucciarelli, S. (2003). Taraxacum officinale as a food source. Plant Foods for Human Nutrition, 58(3), 1-10.
  26. Nayak, R. & Xu, J. P. (2009). Effects of sertraline hydrochloride and fluconazole combinations on Cryptococcus neoformans and Cryptococcus gattii. Mycology, 1(2), 99-105.
  27. Amin, M. M. & Sawhney, S. (2016). Antimicrobial activity of various extracts of Taraxacum officinale. Journal of Microbial & Biochemical Technology, 8(3), 210-215.
  28. Kenny, O., Brunton, N. P., Walsh, D., Hewage, C. M., McLoughlin, P. & Smyth, T. J. (2015). Characterisation of antimicrobial extracts from Dandelion Root (Taraxacum officinale) using LC-SPE-NMR. Phytother Res., 29(4), 526-532.
  29. Kenny, O., Smyth, T. J., Walsh, D., Kelleher, C. T., Hewage, C. M. & Brunton, N. P. (2014). Investigating the potential of under-utilised plants from the Asteraceae family as a source of natural antimicrobial and antioxidant extracts. Food Chem., 161, 79-86.
  30. Bharathi, T., Kolanjinathan, K. & Saranraj, P. (2014). Antimicrobial activity of solvent extracts of Ocimum sanctum, Azadirachta indica and Phyllanthus amarus against clinical pathogens. Global Journal of Pharmacology, 8(3), 294-305.
  31. Sivareddy, B., Reginald, B. A., Sireesha, D., Samatha, M., Reddy, K. H. & Subrahamanyam, G. (2019). Antifungal activity of solvent extracts of Piper betle and Ocimum sanctum Linn on Candida albicans: An in vitro comparative study. J Oral Maxillofac Pathol., 23(3), 333-337.
  32. Khan, R., Islam, B., Akram, M., Shakil, S., Ahmad, A., Ali, S. M., et al. (2009). Antimicrobial Activity of Five Herbal Extracts Against Multi Drug Resistant (MDR) Strains of Bacteria and Fungus of Clinical Origin. Molecules, 14(2), 586-597.
  33. Teka, A., Rondevaldova, J., Asfaw, Z., Demissew, S., Van Damme, P., Kokoska, L., et al. (2015). In vitro antimicrobial activity of plants used in traditional medicine in Gurage and Silti Zones, south central Ethiopia. BMC Complem Altern M., 15(286).
  34. Bisi-Johnson, M. A., Obi, C. L., Samuel, B. B., Eloff, J. N. & Okoh, A. I. (2017). Antibacterial activity of crude extracts of some South African medicinal plants against multidrug resistant etiological agents of diarrhoea. BMC Complem Altern M., 17(321).
  35. Soliman, S. S. M., Semreen, M. H., El-Keblawy, A. A., Abdullah, A., Uppuluri, P. & Ibrahim, A. S. (2017). Assessment of herbal drugs for promising anti-Candida activity. BMC Complem Altern M., 17(257).
  36. Elisha, I. L., Botha, F. S., McGaw, L. J. & Eloff, J. N. (2017). The antibacterial activity of extracts of nine plant species with good activity against Escherichia coli against five other bacteria and cytotoxicity of extracts. BMC Complem Altern M., 17(133).
  37. Altinyay, C., Eryilmaz, M., Yazgan, A. N., Yilmaz, B. S. & Altun, M. L. (2015). Antimicrobial activity of some Alnus species. Eur Rev Med Pharmaco., 19(23), 4671-4674.
  38. Altemimi, A., Lakhssassi, N., Baharlouei, A., Watson, D. G. & Lightfoot, D. A. (2017). Phytochemicals: Extraction, isolation, and identification of bioactive compounds from plant extracts. Plants-Basel., 6(4).
  39. Kim, Y. J., Kim, J. H. & Rho, J. Y. (2019). Antifungal activities of Streptomyces blastmyceticus strain 12-6 against plant pathogenic Fungi. Mycobiology, 47(3), 329-334.
  40. Gould, I. M. (2009). Antibiotic resistance: the perfect storm. Int J Antimicrob Ag., 34(Suppl 3), S2-S5.
  41. Savoia, D. (2012). Plant-derived antimicrobial compounds: alternatives to antibiotics. Future Microbiology, 7(8), 979-990.
  42. Gould, I. M. (2007). Antimicrobials: an endangered species? Int J Antimicrob Agents., 30(5), 383-384.
  43. Fischbach, M. A. & Walsh, C. T. (2009). Antibiotics for emerging pathogens. Science, 325(5944), 1089-1093.
  44. Martinez, J. L., Rojo, F. & Vila, J. (2011). Are nonlethal targets useful for developing novel antimicrobials? Future Microbiol., 6(6), 605-607.
  45. Wise, R. & Need, B. W. P. U. (2011). The urgent need for new antibacterial agents. J Antimicrob Chemoth., 66(9), 1939-1940.
  46. Hoque, M. M., Rattila, S., Shishir, M. A., Bari, M. L., Inatsu, Y. & Kawamoto, S. (2012). Antibacterial activity of ethanol extract of Betel leaf (Piper betle L.) against some food borne pathogens. Bangladesh Journal of Microbiology, 28(2), 58-63.
  47. Kim, J. Y., Yi, Y. S. & Lim, Y. H. (2009). Biological and antifungal activity of herbal plant extracts against Candida species. Korean Journal of Microbiology and Biotechnology, 37(1), 42-48.
  48. Cwikla, C., Schmidt, K., Matthias, A., Bone, K. M., Lehmann, R. & Tiralongo, E. (2010). Investigations into the antibacterial activities of phytotherapeutics against Helicobacter pylori and Campylobacter jejuni. Phytother Res., 24(5), 649-656.
  49. Sohail, Iqbal, Z., Afzal, M., Afzal, A., Rahman, I. U., Shad, S., et al. (2014). In vitro antibacterial study of Taraxacum officinale leaves extracts against different bacterial pathogenic strains. Journal of Pharmacognosy and Phytochemistry, 3(2), 15-17.
  50. Qiao, H. & Sun, T. J. (2014). Antibacterial activity of ethanol extract and fractions obtained from Taraxacum mongolicum flower. Research Journal of Pharmacognosy, 1(4), 35-39.
  51. Kenny, O., Smyth, T. J., H. CM & Brunton, N. P. (2014). Antioxidant properties and quantitative UPLC-MS/MS analysis of phenolic compounds in dandelion (Taraxacum officinale) root extracts. Free Radicals and Antioxidants, 4(1), 55-61.
  52. Wang, H. B. (2014). Cellulase-assisted extraction and antibacterial activity of polysaccharides from the dandelion Taraxacum officinale. Carbohyd Polym., 103, 140-142.
  53. Khan, A. S., Arif, K., Munir, B., Kiran, S., Jalal, F., Qureshi, N., et al. (2019). Estimating total phenolics in Taraxacum officinale (L.) extracts. Pol J Environ Stud., 28(1), 497-501.
  54. Greenwell, M. & Rahman, P. K. S. M. (2015). Medicinal plants: Their use in anticancer treatment. Int J Pharm Sci Res., 6(10), 4103-4112.
  55. Unnati, S., Ripal, S., Sanjeev, A. & Niyati, A. (2013). Novel anticancer agents from plant sources. Chin J Nat Medicines., 11(1), 16-23.
  56. Wang, Z. G. & Ren, J. (2002). Current status and future direction of Chinese herbal medicine. Trends Pharmacol Sci., 23(8), 347-348.
  57. Hong, M., Li, S., Tan, H. Y., Wang, N., Tsao, S. W. & Feng, Y. B. (2015). Current Status of Herbal Medicines in Chronic Liver Disease Therapy: The Biological Effects, Molecular Targets and Future Prospects. Int J Mol Sci., 16(12), 28705-28745.
  58. Sunita, K., Kumar, P., Khan, M. A., Sadaf Husain, S. A. & Singh, D. K. (2017). Anthelminthic/larvicidal activity of some common medicinal plants. European Journal of Biological Research, 7(4), 324-336.
  59. Somerville, V., Moore, R. & Braakhuis, A. (2019). The effect of Olive leaf extract on Upper Respiratory Illness in high school thletes: A randomised control trial. Nutrients, 11(2).

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