Extreme Small, Small and Large Sizes Admixture Nanosilver Fresh Made Magnetic Stirring Colloidal Solution with Lowest, Lower and Higher Dose Repeated Oral Gavages Experiment on Pregnant Swiss Albino Mice and Fetuses: A Toxic and Teratogenic Hippocampal Response Leads to Altered and Disambiguated Psychic State

Pani Jyoti Prakash^1*, Philip Chandra, X.¹, Gupta Rashmi², Pani Sankarsan³, Singh Royana⁴

¹Department of Anatomy Mahatma Gandhi Mission Medical College & Research Institute, Pilliarkuppam, Pondicherry
²Department of Bioinformatics University of North Dakota, United States of America, Post Doctoral Fellowship stream
³Progressive Universal Society, Bharatpur, Rajasthan, Jaipur, India
⁴Department of Anatomy Institute of Medical Sciences Banaras Hindu University Varanasi, Uttar Pradesh

Dr. Pani Jyoti Prakash, Department of Anatomy Mahatma Gandhi Mission Medical College & Research Institute, Pilliarkuppam, Pondicherry.

Keywords: Larger Size Particle Colloidal Silver Nano Teratogenicity; Smaller Size Particle Colloidal Silver Toxicity; Altered and Distorted Hippocampus Response; Fresh AgNps Colloidal Cool Solution of Admixture Sizes

Abstract

The bad impact of daily prolonged administration of silver nanoparticle colloidal solution on cognitive function of Pregnant Swiss Albino mice and their fresh born fetuses were studied. The over building up of nano metallic silver in whole hippocampus tissue and residual part of mice brain was investigated by keane observation of high precise and representative psychic state and its analysis and the meticulous review of histological study were conducted in the present research. Some of the main result of this comprehensive study is description of hippocampus. Stockpiling of nano metallic silver in the pregnant mice mature hippocampus and fetal developing hippocampus inside brain lead to integrity violation of the CA1, CA2, CA3 and CA4 region caused long term contextual memory impairment with visible changes in psychic behavior and cognitive hindrance. This leads to altered psychic state and psychosomatic disorder. Usually 3 types of sizes of nano silver metal are available in market extreme small size (0.25 to 1nm sizes ranges), moderate small or medium size(1.25 to 20.38nm sizes ranges) and larger size(20.75 to 100.55nm sizes ranges) where sizes were focused on the external aspect of package after releasing from manufacturing company. The present study aims to investigate the effect of prenatal exposure to small and large size AgNps on cognition and altered hippocampus response. We commenced serial daily morning (9AM) once repeated oral gavages ingestion experiment with magnetic stirring and cooling fresh made AgNps solution of above 3 sizes on pregnant Swiss Albino mice and their freshly born fetuses (10 from each group treated and sham control and from 6 to 19 gestational days(pregnant mother) and 1month (fetuses after delivery)) and found both sufferers of the same (Toxic and terato genic hippocampus response which is directly dependant on size and dose) after nano silver metal crosses blood brain barrier reaching hippocampus and held inside such white matter tissue.

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